Wednesday April 1, 2009
12 PM
L504 - 80 East Concord Street (MED)

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"Nanomedicine Seminar I - Biosensing: Label-free Microarrays for proteomics and genomics"
Prof. M. Selim Ünlü
Boston University

The Center for Nanoscience and Nanobiotechnology at Boston University has strong research efforts in biosensing, biomaterials, and other emerging nanomedicine areas. In this talk, we will give a general overview of key drivers and applications in nanomedicine, then describe the current research efforts at the BU Charles River Campus in mechanical, electrical and optical biosensors, nanoplasmonics, and microfluidics for point-of-care diagnostics. The primary focus of the seminar will be on biosensing based on optical interference. We have utilized basic principles of optical interference and resonance in biological applications demonstrating label-free sensing of protein binding in a high-throughput micro-array format. Direct monitoring of primary molecular binding interactions without the need for secondary reactants would markedly simplify and expand applications of high-throughput label-free detection methods. We developed a simple interferometric technique – Spectral Reflectance Imaging Biosensor (SRIB) – that monitors the optical phase difference resulting from accumulated biomolecular mass. Dynamic measurements were made at ~10 pg/mm2 sensitivity. We have also demonstrated simultaneous detection of antigens and antibodies in solution using corresponding probes on the SRIB surface as well as label-free measurements of DNA hybridization kinetics. Applications range from protein microarrays for diagnostics and infectious diseases to direct detection of viruses; from point-of-care genotyping to transcription factor/promoter discovery. Our aim is to generate excitement in the BU Medical Campus community and identify collaborative opportunities driven by clinical needs. This seminar is first of the 3 seminar series on applications of nanotechnology in medicine. Upcoming seminars will focus on cardiovascular diseases and cancer and cell growth.

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