Friday July 8, 2005
9 AM - 10 AM
ENG Z-50 Auditorium

Office Hours:
Friday July 8, 2005
3:30 PM - 4:30 PM
ENG B-16

"Spectral Self Interference Microscopy"
Prof. Bennett Goldberg
Boston University

Abstract: Interferometric microscopy can be loosely defined to include any microscopy where counter or co-propagating waves in either the excitation or emission channel, or both, interfere to assist in spatial or spectral resolution enhancement. There are currently many forms of interferometric microscopy that have been developed over the past decade. This talk will review the current status of interferometric microscopy as applied to biological systems. We will compare standard confocal microscopy with 4-Pi microscopy, including the various forms of 4-pi, as well as I5M, sw-TIRF, patterned excitation microscopy, and self-interference microscopy. We will present examples from our work in 4-pi and the work of Stefan Hell, and discuss new directions the field is moving in. We will present recent results that we have obtained on spectral self-interference microscopy, where the orientation of ss- and ds-DNA is determined with sub-nanometer accuracy.